Fig. 2

Validation of the amplification system. A Schematic diagram of the circRNA synthesis and RT-RCA preamplification process; (B) 1% agarose electrophoresis image for the stability of the circRNA by RNase digestion. Lane M: DL5000 DNA marker, lane 1: linear RNA; lane 2: linear RNA obtained after in vitro transcription, lane 3: RNase R digests linear RNA, lane 4: circRNA, lane 5: RNase R digests circRNA; (C)1% agarose electrophoresis image for the RT-RCA process. Lane M:DL5000 DNA marker, lane 1:circRNA, lane 2: RT-RCA primer, lane 3–5: RT-RCA reaction for 1 h, 1.5 h and 2 h; (D) Partial sequencing results of PCR products. The emphasis in the figure is the backsplice junction of circMUC16