Fig. 4

Assessment of Immune Modulation by Flow Cytometry and Cytokine Analyses: A. The variation in immune cell populations within peripheral blood, as determined by flow cytometry. Color depicts the log2 fold-change from baseline at each defined time point (refer to Fig. 1 for time points). Red indicates an increase, blue a decrease in cell population frequency, with significant alterations marked by an ‘X’ (FDR corrected p-value < 0.05). B. Change from baseline in the CD4+/CD8 + cell ratio in peripheral blood, indicating shifts towards either T helper cells (positive values) or cytotoxic cells (negative values). Continuous lines connect sequential time point samples from individual patients, highlighting specific cases of interest in color. Boxplots aggregate data at each time point. C, D. Changes in soluble CD25 (sCD25) and TNFα levels from baseline in peripheral blood. The visualization follows the format of Panel B